FM Minimal Medium Size:1kg

FM Minimal Medium Size:1kgThis media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to

Tryptone 8 Yeast extract 5 NaCl 5 Agar 15 Final pH 7

Casamino acids 17

2 at 25°C Suspend 20

2 at 25°C Blood Agar Base is a non-selective general purpose medium widely employed for the growth of pathogenic and non-pathogenic bacteria

Luria Agar

6 (20 mN) Tryptone 20 Yeast extract 5 NaCl 0

These Amino acids form a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants

0  M  242

Process Biochemistry

glycerol is not fermented to acetic acid

MOPS (3-(N-morpholino)propanesulfonic acid

The FM drop-out mixture