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NZ Top AgarFormula g l Tryptone 10 NaCl 5 MgSO4 anhydrous 0. 98 Agar 7 Final pH 7. 0 0. 2 at 25C Suspend 23 gram powdered medium in 1 litre distilled water Store dry at room temperature.

SIH medium is an improved version of FM medium

has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate

CAS 114-07-8

Glucose 20 Potato extract 4 Suspend 24 gram powdered medium in 1 litre distilled water

Assay (calculated on anhydrous) Min

Character Fine white powder Solubility Freely soluble in water Specific Optical Rotation 104 ~ 121 Water <6% Absorbance 570 nm <0

manganese (Mn)

1% Store at 2 - 8°C in dark

LoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long-term culturing

Yeast extract 10 Peptone 20 Galactose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water

Tryptone 10 Yeast extract 5 NaCl 10 Agar 15 Final pH 7

95 The materials used in the production are in compliance with the European Directive 75/318/EEC as amended by Directive 1999/82/EC

Exchange/Return Notes